For Research Use Only
PrepaCyte-WBC is a ready-to-use liquid reagent that efficiently separates white blood cells (WBC) – including stem cells – from peripheral blood, umbilical cord blood or bone marrow for research applications.
Negative Selection Process
PrepaCyte-WBC’s red cell depletion process leaves desired cells unmodified while depleting nearly 99 percent of red blood cells (RBC).
PrepaCyte-WBC can be used to remove RBC and isolate WBC from blood for a variety of uses such as culturing, purification, depletion, enrichment, etc. Contact BioE for further information on technical applications of this product.
Ease Of Use
This non-density gradient separation medium is extremely easy to use. Simply mix PrepaCyte-WBC with an equal volume of sample and allow the mixture to sit undisturbed for 30 minutes, and then remove the plasma and centrifuge to concentrate the cells.
ActiCyte-TC Media Kit contains a cell activation media designed to induce expression of cytokine receptors and secretion of cytokines in human leukocyte cell cultures. It is designed to facilitate the study of T-lymphocyte activation biology by providing a rapid and simple methodology of cell preparation by utilizing anti-CD3 antibody, hIL-1 alpha and hIL-2 to activate human T-lymphocytes. It includes activation medium and a basal medium for the establishment of parallel control cultures. For research use only.
Proof of Performance
Typical results of cytokine secretions at 20 h of culture on PBMCs and purified T-cells (PrepaCyte®) using various activation methods including ActiCyte-TC. Secretions measured in pg/mL using flow cytometry-based immunoassay kits. (Collins, D. 2000. Journal of Immunological Methods. 243: 125-145)
VitaLyse is a reagent system designed to lyse erythrocytes in peripheral blood, lymph nodes, bone marrow, umbilical cord blood and other biological specimens. Leukocytes recovered after lysing treatment are fully viable and ready for flow cytometric immunophenotypic analysis, fluorescence-activated cell sorting or tissue culture. VitaLyse maintains complete viability and biological function of leukocytes without activation. VitaLyse has been shown to be especially useful in removing erythrocytes from bone marrow samples in the preparation of fragile and immature cells for long-term colony assays and in the preparation of T-cells for long-term activation cultures. If desired, cells for flow cytometric analysis can also be fixed (included in kit) and stored at 2-8° C for later analysis. VitaLyse is ideal for use in conjunction with PrepaCyte separated samples. For research use only.
Proof of Performance
Light scatter of a normal ACD blood sample processed with VitaLyse
CD45 versus CD14 showing 98% purity in the lymphocyte gate
CD45 gating of a normal ACD blood sample processed with VitaLyse
CD45 versus CD14 staining of leukocytes
Designed to fix and permeabilize cells allowing detection of intracellular antigens by immunoflourescence. PermaCyteÔ reagents first stabilize cell structure by fixation and then permeabilize the cell membrane by detergent. PermaCyteÔ utilizes a detergent that causes permanent holes in the cell membrane. This allows better access to internal antigens by fluorescently-labeled antibodies and better washing of unbound antibody resulting in superior signal-to-noise.
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A cell preservation technology based on the PreserveTM system. This system consists of 2 buffers, used in sequence, which stabilizes the morphology, protein structure, and integrity of DNA and RNA (including mRNA) of adherent-dependent and suspension cells at refrigerator temperatures for a month without fixation. The cells are suitable for immunohistochemistry, flow cytometry and in situ hybridization analysis. This system enables the development of specimens that are quickly accessible for extended and repeated analysis. This system would also be useful in the preservation of tissue samples for transportation of diagnostic and forensic applications.
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